Used BioTek Epoch™ Microplate Spectrophotometer

The Used BioTek Epoch™ Microplate Spectrophotometer​ is a highly reliable, cost-effective, and filter-free UV-Vis absorbance detection platform, originally manufactured by BioTek (now part of Agilent Technologies). Acquiring this instrument in certified used or refurbished condition offers laboratories an exceptional opportunity to access premium monochromator-based optical performance at a fraction of the cost of a new unit, without compromising on data quality or instrument durability. Designed with a sophisticated grating-based monochromator, the Epoch eliminates the traditional limitations and ongoing expenses associated with filter-based readers. Researchers can instantly select any wavelength from 200 to 999 nm in precise 1 nm increments directly from the software interface, making it incredibly versatile for spectral scanning, multi-wavelength endpoint readings, and kinetic assay development without needing to purchase, store, or physically swap optical filters.

A major advantage of the BioTek Epoch, particularly for labs handling precious samples, is its seamless compatibility with the optional Take3 and Take3 Trio Micro-Volume Plates. When equipped with a Take3 plate, this microplate spectrophotometer transforms into a high-precision nucleic acid and protein quantification system capable of accurately measuring samples as small as 2 µL. This eliminates the need for sample dilution and saves significant amounts of valuable DNA, RNA, or purified protein. Furthermore, the instrument accommodates standard 6-well to 384-well microplates, making it a true workhorse for a vast array of quantitative and qualitative colorimetric assays. Common applications include ELISA (endpoint and kinetic), BCA/Bradford/Lowry protein assays, microbial growth curves (OD600), enzyme kinetics, cell viability assays (MTT, XTT, MTS), and general absorbance-based screening.

The Epoch is powered by a long-life Xenon flash lamp and utilizes a high-sensitivity photodiode detector to ensure low stray light and excellent photometric accuracy (±1% at 0-2 OD) and precision. Controlled via the industry-standard Gen5 Data Analysis Software, the system offers intuitive protocol setup, automated pathlength correction (normalizing microplate reads to a standard 1 cm pathlength for direct concentration calculation), and robust curve-fitting tools. Its compact benchtop design (approx. 30.5 x 31.7 x 19.5 cm) and lightweight build (under 7 kg) allow it to fit easily into nearly any lab environment, including inside biosafety cabinets for live-cell assay reading. For labs requiring higher throughput, the 'EpochR' configuration is compatible with BioTek's BioStack microplate stackers and third-party robotic automation systems.

Purchasing a used BioTek Epoch unit typically involves instruments that have been professionally inspected, tested, and certified to meet original manufacturer specifications. These units often show minor cosmetic wear but deliver full optical and mechanical functionality, providing an ideal solution for startup labs, teaching facilities, or established research groups looking to expand their instrumentation capacity economically. With its robust solid-state design (featuring no moving optical parts), the Epoch is known for long-term reliability and minimal maintenance requirements, ensuring that even a pre-owned unit will deliver consistent, publication-quality data for years to come.

Technical Specifications