Used Molecular Devices SpectraMax® ABS Absorbance Microplate Reader

The Molecular Devices SpectraMax® ABS Absorbance Microplate Reader​ is a high-performance, filter-free absorbance detection platform designed to deliver the flexibility of monochromator-based systems with the cost-efficiency expected by modern research and clinical laboratories. Bridging the gap between basic filter-based readers and high-end multi-mode instruments, the SpectraMax ABS focuses exclusively on precision absorbance measurements, making it an ideal solution for Endpoint, Kinetic, and Spectral Scan assays. Utilizing a high-quality grating monochromator, the instrument allows users to select any wavelength from 340 to 850 nm in 1 nm increments without the need to purchase, store, or change physical filters. This "filterless" flexibility enables researchers to easily optimize assays for various chromogenic substrates and dyes, covering essential applications such as ELISA, protein quantification (BCA, Bradford, Lowry), cell viability (MTT, XTT, MTS), enzyme kinetics, and microbial growth curves.

A standout feature of the SpectraMax ABS is its integration of Molecular Devices’ patented PathCheck® Sensor​ technology. This innovative, temperature-independent sensor measures the fluid depth (optical pathlength) in each microplate well, automatically normalizing the absorbance reading to a standard 1 cm pathlength. This capability effectively converts a 96-well microplate into 96 virtual cuvettes, allowing researchers to calculate concentrations directly using known extinction coefficients without running tedious standard curves. Furthermore, PathCheck technology detects pipetting errors and volume discrepancies between wells, ensuring data integrity and reliability even when sample volumes vary slightly. The reader is equipped with an advanced 8-channel detector head, enabling rapid plate reads (a 96-well plate can be read in as little as 9 seconds), alongside a user-programmable linear shaking mechanism to ensure thorough sample mixing prior to measurements.

Designed with a compact footprint, the SpectraMax ABS fits seamlessly into virtually any laboratory setting, from academic benchtops to high-throughput screening environments. It is fully compatible with industry-leading SoftMax® Pro Software, which offers intuitive protocol setup, powerful data analysis tools, and comprehensive GxP compliance features (including 21 CFR Part 11 options) for regulated environments. The software includes pre-defined templates for common assays and allows for custom calculations and curve fitting. For labs requiring ultra-low volume quantification, the SpectraMax ABS is compatible with the SpectraDrop™ Micro-Volume Microplate, facilitating high-throughput analysis of samples under 5 µL—perfect for precious nucleic acid and protein samples. Additionally, the instrument supports validation routines using the SpectraTest® ABS1 Absorbance Validation Plate with NIST-traceable standards, ensuring the instrument maintains peak performance over time. Combining speed, accuracy, and user-friendly operation, the SpectraMax ABS Absorbance Microplate Reader provides a robust, dependable, and versatile foundation for any lab's absorbance detection needs.

Technical Specifications

Note: For extended UV (190–1000 nm) and 384-well plate/cuvette compatibility, please refer to the SpectraMax ABS Plus model.