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Detailed Introduction
The Thermo Scientific Applied Biosystems QuantStudio 3 Real-Time PCR System is a compact, high-performance quantitative PCR (qPCR) instrument designed for reliable gene expression analysis, genotyping, pathogen detection, and a wide range of molecular biology applications. Developed by Thermo Fisher Scientific under its Applied Biosystems platform, the QuantStudio 3 combines precision thermal cycling, advanced optical detection, and intuitive software to deliver consistent and reproducible real-time PCR results.
The system is designed for both routine and research-grade molecular diagnostics laboratories. It offers a user-friendly touchscreen interface, simplified workflow, and cloud-enabled data management capabilities, making it suitable for users of all experience levels. Its compact footprint allows it to fit easily into space-limited laboratories without compromising performance.
The QuantStudio 3 utilizes five-color fluorescence detection technology, enabling multiplex analysis of up to five targets in a single reaction. This significantly improves throughput and reduces reagent consumption. The system’s optical calibration is factory-optimized, ensuring consistent sensitivity and accuracy across runs.
With its Veriflex temperature block technology, the system provides flexible throughput options, allowing users to run 96-well plates efficiently. The thermal system ensures rapid heating and cooling rates, improving experimental efficiency while maintaining precise temperature control.
Typical applications include:
Gene expression analysis
SNP genotyping
Pathogen detection (viral, bacterial, fungal)
miRNA analysis
Pharmacogenomics research
Food safety and environmental testing
The integrated software platform allows experiment setup, real-time monitoring, and post-run analysis in a streamlined workflow. Data can be easily exported or shared through network integration, supporting modern laboratory data management systems.
Overall, the QuantStudio 3 is widely recognized for its balance of performance, ease of use, and affordability, making it an ideal choice for clinical research, academic labs, and industrial molecular biology applications.
| Item | Specification |
|---|---|
| Product Name | Applied Biosystems QuantStudio 3 |
| Manufacturer | Thermo Fisher Scientific |
| System Type | Real-Time PCR (qPCR) System |
| Block Format | 96-well block |
| Detection Channels | 5-color fluorescence detection |
| Optical System | LED excitation with CCD detection |
| Temperature Range | 4°C to 100°C |
| Heating/Cooling Rate | Up to ~3–6°C/sec (approx.) |
| Dynamic Range | Up to 10 orders of magnitude |
| Run Time | Fast and standard PCR modes supported |
| Software | QuantStudio Design & Analysis Software |
| Interface | Touchscreen + PC software control |
| Connectivity | USB / Ethernet network |
| Data Analysis | Relative quantification, melt curve analysis |
| Sample Volume | 10–100 µL typical |
| Applications | Gene expression, genotyping, pathogen detection |
| Operating System | Windows-based control software |
| Instrument Size | Compact benchtop design |
| Power Supply | Standard laboratory electrical input |
The QuantStudio 3 qPCR system provides an excellent balance of sensitivity, ease of use, and multiplex capability, making it a widely adopted platform in molecular biology and diagnostic laboratories worldwide.
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