Thermo Scientific NanoDrop One Microvolume UV-Vis Spectrophotometer

The Thermo Scientific NanoDrop One Microvolume UV-Vis Spectrophotometer represents the next generation of the world-renowned NanoDrop series, designed to deliver fast, accurate, and intelligent quantification of nucleic acids and proteins with unparalleled ease of use. Requiring only 1 to 2 µL of sample pipetted directly onto the stainless-steel and quartz-fiber pedestal, the instrument utilizes a proprietary sample retention system where lowering the arm forms a liquid column with an auto-ranging pathlength of 0.030 to 1.0 mm. This eliminates the need for cumbersome cuvettes, capillaries, or dilutions, even for highly concentrated samples up to 27,500 ng/µL for dsDNA. Powered by a xenon flash lamp and a 2048-element CMOS linear image sensor, it captures full-spectrum data from 190 to 850 nm in approximately 8 seconds. The standout feature of the NanoDrop One is the integrated Acclaro Sample Intelligence technology, which analyzes the entire absorption profile to identify potential contaminants—such as proteins in DNA prep or phenol residues—and automatically provides corrected concentration values, ensuring higher confidence in downstream applications like PCR or sequencing.

Operating as a fully independent workstation, the NanoDrop One features a high-definition, glove-compatible 7-inch touchscreen interface powered by an internal Android-based system, removing the immediate necessity for an external PC, though Ethernet and PC control software options remain available. The intuitive interface grants immediate access to pre-loaded applications for DNA, RNA, oligos, purified proteins (A280/A205), and various colorimetric assays including BCA, Bradford, and Pierce 660, alongside custom method creation. Users benefit from instantaneous results, automatic blanking, and the calculation of purity ratios such as A260/A280 and A260/A230 directly on the device. Furthermore, an onboard Learning Center provides immediate access to technical support documents and educational animations, facilitating seamless operation for all users. Connectivity is robust, featuring USB, Ethernet, Bluetooth, and Wi-Fi options for effortless data export, remote viewing, and integration into laboratory information management systems, with an optional Security Suite available to support compliance with FDA 21 CFR Part 11 regulations for regulated environments.

Highly versatile and compact, with a footprint of only 20 x 25.4 x 32.3 cm and a weight of 3.6 kg, the NanoDrop One fits easily on any crowded benchtop. It serves as an indispensable tool across molecular biology, biochemistry, microbiology, and pharmaceutical research, routinely handling tasks such as quantifying extracted genomic DNA or RNA, assessing protein concentration during purification workflows, measuring cell density via OD600, and evaluating nanoparticle suspensions. The ability to differentiate DNA from co-purified RNA and correct for background contamination on-the-fly significantly streamlines workflow and reduces costly experimental errors. Whether utilized for high-throughput screening preparation, quality control in core facilities, or daily sample checks in academic laboratories, the NanoDrop One combines the trusted reliability of the NanoDrop legacy with modern intelligent software and standalone convenience, making it a critical asset for any life science laboratory focused on efficiency and data integrity.