Agilent 1260 infinity III HPLC system

The Agilent 1260 Infinity III HPLC System is a robust and flexible high-performance liquid chromatography platform designed for reliable routine analysis, quality control, pharmaceutical research, chemical testing, food analysis, environmental testing, and other analytical laboratory applications. As part of the Agilent InfinityLab LC family, the 1260 Infinity III provides a broad selection of modules that can be configured according to different analytical requirements.

One of the key advantages of the 1260 Infinity III is its modular architecture. Laboratories can select binary, quaternary, or isocratic pumping configurations, together with different autosamplers, column compartments, and detectors. This flexibility allows users to build an HPLC system for routine analytical work or configure the system for more demanding applications and entry-level UHPLC workflows. Agilent specifies a maximum system operating pressure of up to 600 bar for the standard 1260 Infinity III LC system, while the specific pressure capability depends on the selected module.

The system offers a broad settable flow range, with configurations supporting approximately 0.001–10 mL/min. The 1260 Infinity III Quaternary Pump can deliver flow rates up to 10 mL/min, making it suitable for standard analytical and selected higher-flow applications. The Binary Pump supports flow rates up to 5 mL/min and is particularly suitable for fast and precise gradient applications.

Sample introduction is another important strength. Depending on configuration, the system supports injection volumes from 0.1 to 100 µL, with extended injection options available for applications requiring larger volumes. The 1260 Infinity III Multisampler can accommodate vials and microplates and, according to Agilent, can handle up to 6,144 samples in its maximum configuration.

For temperature-controlled separations, the 1260 Infinity III Multicolumn Thermostat can accommodate up to four columns and provides temperature control up to 85 °C, depending on configuration. This makes it useful for applications where retention time and selectivity depend strongly on column temperature.

The detector portfolio is also extensive. Users can select diode-array, variable-wavelength, multiple-wavelength, fluorescence, refractive-index, and evaporative light-scattering detection according to their analytical needs. For example, the 1260 Infinity III Diode Array Detector HS provides UV spectral detection from 190 to 640 nm with a data rate of up to 120 Hz, while the wide-range DAD version covers 190 to 950 nm.

The 1260 Infinity III is therefore suitable for laboratories seeking a dependable and configurable HPLC platform. Its modular design also makes it possible to combine selected new modules with existing Agilent HPLC equipment, potentially helping laboratories expand analytical capabilities while managing equipment investment.

Agilent 1260 Infinity III HPLC System Specifications

Specifications are configuration-dependent. Users should confirm the exact module model and configuration before purchase or installation.

Applications

The Agilent 1260 Infinity III HPLC System can be used for a wide range of applications, including pharmaceutical quality control, impurity analysis, assay testing, food and beverage analysis, environmental testing, chemical research, academic research, and routine analytical testing.

For laboratories purchasing a used or refurbished Agilent 1260 Infinity III, it is particularly important to verify the pump model, detector configuration, autosampler, column compartment, firmware/software compatibility, operating pressure, leak condition, and chromatographic performance before purchase.

Overall, the Agilent 1260 Infinity III combines modularity, reliable routine performance, broad detector selection, flexible solvent delivery, and scalable configuration, making it a practical HPLC platform for laboratories with diverse analytical requirements.