Agilent 1260 infinity I HPLC system

The Agilent 1260 Infinity HPLC stack is a fully modular, high-performance liquid chromatography system designed for routine quantitative analysis, method development, and quality control in pharmaceutical, food safety, environmental, and life science laboratories. This configuration integrates a G1322A online vacuum degasser, a G1312B binary pump, a G1367E high-performance autosampler (HiP-ALS) with G1330B thermostat, a G1314F variable wavelength detector (VWD), and a dedicated PC running Agilent OpenLAB CDS ChemStation or MassHunter software, providing a complete turnkey analytical solution. The G1322A inline degasser utilizes a four-channel tubular membrane under controlled vacuum to remove dissolved gases from mobile phase solvents at flow rates up to 10 mL/min per channel, preventing bubble formation in the pump heads and baseline noise in the detector. The G1312B binary pump employs a dual-piston in-series design with active inlet valves and high-pressure gradient mixing (A and B channels blended post-compression) to deliver pulse-free flow from 0.001 to 10 mL/min with ≤0.075% RSD precision and a typical delay volume of ~650 µL (standard) or ~120 µL (low-dwell option), operating safely up to 600 bar (8,700 psi), which makes it compatible with sub‑2 µm UHPLC columns for faster, higher-resolution separations. The G1367E HiP-ALS is a high-throughput autosampler featuring a dual-needle overlapping injection design and a metal-free metering pump for precise loop filling; it accommodates 2 mL vials, 96‑well, and 384‑well microplates with programmable needle wash protocols, delivering injections from 0.1 to 100 µL with <0.25% RSD repeatability and typically <0.05% carryover, enabling unattended overnight sequences of thousands of samples. The attached G1330B 1290 Infinity Thermostat provides Peltier-based column heating and optional cooling (ambient –10 °C to 80 °C or 4–80 °C with cooling option), holding temperature within ±0.15 °C to ensure retention time reproducibility and support dual-column switching. Detection is performed by the G1314F VWD, which incorporates a dual-beam optical layout and a Max-Light high-throughput flow cell (10 mm pathlength, 13 µL volume, 400 bar pressure rating) covering 190–600 nm with a noise level typically <±0.75 × 10⁻⁵ AU at 254 nm, a drift <1 × 10⁻⁴ AU/hr, and data collection rates up to 80 Hz, suitable for trace impurity quantitation and routine assay work. The entire stack communicates via Agilent CAN bus and is operated through the PC workstation, which offers instrument control, multi-level calibration curves, 3D overlay viewing, audit trails, and compliance with 21 CFR Part 11 for GMP/GLP environments. Compact, robust, and easily field-upgradable (e.g., to DAD or fluorescence detector), the Agilent 1260 Infinity binary-gradient HPLC system balances analytical versatility, sensitivity, and throughput for demanding production and research labs alike.