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Detailed Introduction
The BD LSR Fortessa Cell Sorter is a high-performance multi-laser flow cytometry platform designed for advanced cellular analysis applications in immunology, molecular biology, oncology research, stem cell studies, pharmaceutical development, and clinical research. Developed by BD (Becton, Dickinson and Company), the LSR Fortessa series is recognized for its powerful fluorescence detection capability, high sensitivity, and flexible experimental configuration.
This system includes a four-laser configuration, HTS (High Throughput Sampler), computer workstation, BD FACSDiva 9.0 software with dongle license, sheath cart, and additional supplies. It provides researchers with a complete solution for multi-parameter flow cytometry analysis and high-throughput sample processing.
The four-laser optical configuration allows simultaneous excitation and detection of multiple fluorescent markers. Typical laser configurations include violet (405 nm), blue (488 nm), yellow-green (561 nm), and red (640 nm) lasers. This enables researchers to analyze complex biological samples using multiple fluorescent antibodies or molecular probes. The system is widely used for immune cell phenotyping, apoptosis studies, cell cycle analysis, intracellular cytokine detection, and biomarker research.
One of the major advantages of the BD LSR Fortessa is its excellent sensitivity and resolution. The advanced optical system and detector design provide accurate separation of weak and strong fluorescence signals, allowing researchers to identify rare cell populations and perform detailed cellular characterization. The instrument supports complex multi-color experiments while maintaining reliable data quality and reproducibility.
The integrated HTS (High Throughput Sampler) module improves laboratory efficiency by enabling automated processing of samples from multi-well plates, including 96-well and 384-well formats. This feature is especially valuable for large-scale experiments such as drug screening, antibody evaluation, and population analysis, significantly reducing manual operation time and improving workflow consistency.
The system is operated through BD FACSDiva 9.0 software, a powerful platform designed specifically for BD flow cytometry instruments. FACSDiva provides comprehensive functions for instrument setup, fluorescence compensation, acquisition control, data analysis, and experiment management. The included software dongle provides authorized access and ensures secure operation of the system.
The included PC workstation allows users to control instrument operation and process experimental data. Researchers can create experimental templates, adjust acquisition parameters, monitor real-time signals, and analyze flow cytometry results efficiently.
The sheath cart system provides convenient management of sheath fluid supply and waste collection. Its mobile design improves laboratory flexibility and simplifies routine operation and maintenance. Additional supplies included with the system can help users prepare the instrument for immediate laboratory applications, depending on the specific inventory provided.
The BD LSR Fortessa is suitable for a wide range of applications, including:
Immunophenotyping of immune cells
T-cell and B-cell analysis
Cancer biomarker research
Stem cell characterization
Cell cycle and proliferation studies
Apoptosis detection
Fluorescent protein analysis
Pharmaceutical screening
Basic and clinical biomedical research
For laboratories purchasing refurbished systems, proper inspection of laser performance, optical alignment, detector sensitivity, fluidics condition, software licensing, and performance verification is highly recommended. Regular cleaning, calibration, and quality control procedures are essential for maintaining long-term analytical accuracy.
Overall, the BD LSR Fortessa Four-Laser Cell Sorter with HTS and FACSDiva 9.0 software is a powerful and versatile flow cytometry solution. Its combination of multi-color capability, automated sampling, advanced software control, and reliable performance makes it an excellent choice for research laboratories requiring high-quality cellular analysis.
| Parameter | Specification |
|---|---|
| Product Name | BD LSR Fortessa Cell Sorter |
| Instrument Type | Multi-parameter flow cytometry analysis system |
| Brand | BD (Becton, Dickinson and Company) |
| Laser Configuration | Four-laser system |
| Typical Laser Lines | 405 nm Violet, 488 nm Blue, 561 nm Yellow-Green, 640 nm Red |
| Fluorescence Detection | Multi-color fluorescence detection |
| Application | Cell analysis, immunology, oncology, biotechnology research |
| High Throughput Module | HTS (High Throughput Sampler) |
| Supported Plate Formats | 96-well and 384-well plates (application dependent) |
| Software | BD FACSDiva 9.0 |
| Software License | Dongle-based authorization included |
| Computer System | Included PC workstation |
| Fluidics System | Sheath fluid delivery and waste management system |
| Included Accessory | Sheath cart |
| Additional Items | Extra supplies included |
| Data Acquisition | Automated flow cytometry data collection |
| Data Analysis | Compensation, gating, statistics, experiment management |
| Optical System | Advanced multi-laser excitation and fluorescence detection |
| Sample Type | Cells, biological particles, fluorescently labeled samples |
| Main Applications | Immunophenotyping, cell cycle, apoptosis, biomarker analysis |
| Installation Requirement | Stable laboratory bench and appropriate electrical supply |
| Not Included | Bench and LCD monitors (unless separately specified) |
| Maintenance Requirements | Regular cleaning, fluidics maintenance, calibration, and quality control |
The BD LSR Fortessa Four-Laser Cell Sorter with HTS, PC, FACSDiva 9.0 software, and sheath cart represents a complete and flexible flow cytometry platform for advanced biological research. With powerful multi-color detection, automated sample handling, and professional software control, it provides researchers with accurate, reproducible, and efficient solutions for complex cellular analysis workflows.
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